Separation of selenium compounds by CE-ICP-MS in dynamically coated capillaries applied to selenized yeast samples
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Separation of selenium compounds by CE-ICP-MS in dynamically coated capillaries applied to selenized yeast samples. / Bendahl, Lars; Gammelgaard, Bente.
I: Journal of Analytical Atomic Spectrometry, Bind 19, Nr. 1, 2004, s. 143-148.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Separation of selenium compounds by CE-ICP-MS in dynamically coated capillaries applied to selenized yeast samples
AU - Bendahl, Lars
AU - Gammelgaard, Bente
PY - 2004
Y1 - 2004
N2 - The selenium species in nutritional supplement tablets, based on selenized yeast, were separated by capillary zone electrophoresis using capillaries coated dynamically with poly(vinyl sulfonate) and detected by ICP-MS. Sample pre-treatment consisted of cold-water extraction by sonication and subsequent incubation of the cold-water extract with 6 M hydrochloric acid at 110 degreesC. The total selenium concentration in the cold-water extract was 3.5 mg L-1 and corresponded to 9% of the total selenium content of the tablets. More than 20 different selenium compounds were separated in the cold-water extract within 13 min. The efficiency of the system corresponded to 620 000 theoretical plates. When spiking the sample with available standards, co-migration was observed with selenomethionine and selenocystine-Se-methylselenocysteine-the latter species were not separated. When the cold-water extract was hydrolysed in hot hydrochloric acid, 45% of the selenium migrated within a single peak that co-migrated with selenomethionine. Other peaks co-migrated with trimethylselenonium, Se-methylselenomethionine, and selenocystine-Se-methylselenocysteine, respectively. The precision for the analysis of the aqueous extracts expressed as relative standard deviation (n = 3) on peak heights and areas was in the range 1.4-5.3%. Detection limits were better than 15 mug L-1, corresponding to absolute detection limits less than 250 fg
AB - The selenium species in nutritional supplement tablets, based on selenized yeast, were separated by capillary zone electrophoresis using capillaries coated dynamically with poly(vinyl sulfonate) and detected by ICP-MS. Sample pre-treatment consisted of cold-water extraction by sonication and subsequent incubation of the cold-water extract with 6 M hydrochloric acid at 110 degreesC. The total selenium concentration in the cold-water extract was 3.5 mg L-1 and corresponded to 9% of the total selenium content of the tablets. More than 20 different selenium compounds were separated in the cold-water extract within 13 min. The efficiency of the system corresponded to 620 000 theoretical plates. When spiking the sample with available standards, co-migration was observed with selenomethionine and selenocystine-Se-methylselenocysteine-the latter species were not separated. When the cold-water extract was hydrolysed in hot hydrochloric acid, 45% of the selenium migrated within a single peak that co-migrated with selenomethionine. Other peaks co-migrated with trimethylselenonium, Se-methylselenomethionine, and selenocystine-Se-methylselenocysteine, respectively. The precision for the analysis of the aqueous extracts expressed as relative standard deviation (n = 3) on peak heights and areas was in the range 1.4-5.3%. Detection limits were better than 15 mug L-1, corresponding to absolute detection limits less than 250 fg
M3 - Journal article
VL - 19
SP - 143
EP - 148
JO - Journal of Analytical Atomic Spectrometry
JF - Journal of Analytical Atomic Spectrometry
SN - 0267-9477
IS - 1
ER -
ID: 44288244