Optimization of LC-DRC-ICP-MS for the speciation of selenotrisulfides with simultaneous detection of sulfur and selenium as oxides combined with determination of elemental and isotope ratios

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A LC-DRC-ICP-MS method for the simultaneous detection of selenium and Sulfur in the selenotrisulfides selenocysteineglutathione (Cys-Se-SG) and selenodiglutathione (GS-Se-SG) is described. Both sulfur and selenium are reacted with oxygen in the dynamic reaction cell (DRC) and detected as oxides. The selenotrisulfides were separated applying a 30 rnin gradient liquid chromatographic (LC) method with a formic acid/methanol eluent. The detection limits for sulfur (as (SO+)-S-32-O-16) and selenium (as (SeO+)-Se-80-O-16) in the chromatographic system were 4.0 and 0.2 mu g L-1 (100 and 5 ng in absolute mass units), respectively. S/Se elemental ratios in the 1.9-2.1 range, close to the theoretical value of 2.0, was found for both selenotrisulfides along with accurate (
OriginalsprogEngelsk
TidsskriftJournal of Analytical Atomic Spectrometry
Vol/bind21
Udgave nummer2
Sider (fra-til)201-203
Antal sider3
ISSN0267-9477
StatusUdgivet - 2006

ID: 44287946