Interfacing capillary electrophoresis with inductively coupled plasma mass spectrometry by direct injection nebulization for selenium speciation
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A demountable direct injection high efficiency nebulizer operating at low sample uptake rates was developed and used for coupling of capillary electrophoresis (CE) with inductively coupled plasma mass spectrometry (ICP-MS). When the nebulizer was used for continuous sample introduction, detection limits of 20 and 1 ng L-1 were obtained for Se-82 and Rh-103, respectively, at sample uptake rates of 10-30 muL min(-1), based on three times the standard deviation of blank solution (3 sigma (b), n = 10). The nebulizer was used as part of the interface for coupling of CE with ICP-MS and applied for speciation of aqueous selenium standards. The interface was operated in the self-aspirating mode with a sheath liquid uptake of 10 muL min(-1). The CE-ICP-MS system resulted in baseline separation of selenate, selenite, selenocystine and selenomethionine within a total analysis time of 5.4 min. Detection limits were in the sub mug Se L-1 range, corresponding to absolute detection limits in the range 25-125 fg selenium. Repeatability (n = 6) expressed as relative standard deviations with respect to migration times, peak heights and peak areas were better than 1.6, 6.7 and 6.0%, respectively
Originalsprog | Engelsk |
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Tidsskrift | Journal of Analytical Atomic Spectrometry |
Vol/bind | 16 |
Udgave nummer | 1 |
Sider (fra-til) | 38-42 |
Antal sider | 5 |
ISSN | 0267-9477 |
Status | Udgivet - 2001 |
ID: 44288185