TY - JOUR

T1 - In vitro metabolism and permeation studies in rat jejunum

T2 - organic chromium compared to inorganic chromium

AU - Gammelgaard, Bente

AU - Jensen, K

AU - Steffansen, B

PY - 1999

Y1 - 1999

N2 - The purpose of these studies was to compare the in vitro absorption of two inorganic chromium(III) compounds: chromium chloride and chromium nitrate, with organic chromium(III)-picolinate; and to investigate if any in vitro metabolism of chromium(VI) takes place. The in vitro metabolism studies showed that chromium (VI) was reduced by artificial gastric juice. The reduction followed first order kinetics with a half-life of 23 min. The studies also showed that the chromium picolinate complex was stable in artificial gastric juice for 4 hours. By the rat everted gut sac technique, chromium chloride, chromium nitrate and chromium picolinate penetrated the rat jejunum with 165 +/- 59, 160 +/- 26 and 127 +/- 36 ng chromium per g rat jejunum, respectively, whereas the permeability coefficients (Papp) were 0.7 +/- 0.3, 1.0 +/- 0.4, and 9.6 +/- 2.2 microns/min, respectively. Absorption studies on pig intestine in Ussing chambers showed a nearly total adsorption of chromium(III) by the chambers, resulting in unreliable data.

AB - The purpose of these studies was to compare the in vitro absorption of two inorganic chromium(III) compounds: chromium chloride and chromium nitrate, with organic chromium(III)-picolinate; and to investigate if any in vitro metabolism of chromium(VI) takes place. The in vitro metabolism studies showed that chromium (VI) was reduced by artificial gastric juice. The reduction followed first order kinetics with a half-life of 23 min. The studies also showed that the chromium picolinate complex was stable in artificial gastric juice for 4 hours. By the rat everted gut sac technique, chromium chloride, chromium nitrate and chromium picolinate penetrated the rat jejunum with 165 +/- 59, 160 +/- 26 and 127 +/- 36 ng chromium per g rat jejunum, respectively, whereas the permeability coefficients (Papp) were 0.7 +/- 0.3, 1.0 +/- 0.4, and 9.6 +/- 2.2 microns/min, respectively. Absorption studies on pig intestine in Ussing chambers showed a nearly total adsorption of chromium(III) by the chambers, resulting in unreliable data.

KW - Animals

KW - Cell Membrane Permeability

KW - Chlorides

KW - Chromium

KW - Chromium Compounds

KW - Female

KW - Gastric Juice

KW - Intestinal Absorption

KW - Jejunum

KW - Kinetics

KW - Models, Biological

KW - Nitrates

KW - Oxidation-Reduction

KW - Picolinic Acids

KW - Rats

KW - Rats, Sprague-Dawley

U2 - 10.1016/S0946-672X(99)80028-5

DO - 10.1016/S0946-672X(99)80028-5

M3 - Journal article

C2 - 10445223

VL - 13

SP - 82

EP - 88

JO - Journal of Trace Elements in Medicine and Biology

JF - Journal of Trace Elements in Medicine and Biology

SN - 0946-672X

IS - 1-2

ER -