HPLC-ICP-MS compared with radiochemical detection for metabolite profiling of H-3-bromohexine in rat urine and faeces

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Standard

HPLC-ICP-MS compared with radiochemical detection for metabolite profiling of H-3-bromohexine in rat urine and faeces. / Jensen, B.P.; Gammelgaard, B.; Hansen, S.H.; Andersen, J.V.

I: Journal of Analytical Atomic Spectrometry, Bind 20, Nr. 3, 2005, s. 204-209.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Jensen, BP, Gammelgaard, B, Hansen, SH & Andersen, JV 2005, 'HPLC-ICP-MS compared with radiochemical detection for metabolite profiling of H-3-bromohexine in rat urine and faeces', Journal of Analytical Atomic Spectrometry, bind 20, nr. 3, s. 204-209.

APA

Jensen, B. P., Gammelgaard, B., Hansen, S. H., & Andersen, J. V. (2005). HPLC-ICP-MS compared with radiochemical detection for metabolite profiling of H-3-bromohexine in rat urine and faeces. Journal of Analytical Atomic Spectrometry, 20(3), 204-209.

Vancouver

Jensen BP, Gammelgaard B, Hansen SH, Andersen JV. HPLC-ICP-MS compared with radiochemical detection for metabolite profiling of H-3-bromohexine in rat urine and faeces. Journal of Analytical Atomic Spectrometry. 2005;20(3):204-209.

Author

Jensen, B.P. ; Gammelgaard, B. ; Hansen, S.H. ; Andersen, J.V. / HPLC-ICP-MS compared with radiochemical detection for metabolite profiling of H-3-bromohexine in rat urine and faeces. I: Journal of Analytical Atomic Spectrometry. 2005 ; Bind 20, Nr. 3. s. 204-209.

Bibtex

@article{e7060bf0eb114675b5df295e9acc6d97,
title = "HPLC-ICP-MS compared with radiochemical detection for metabolite profiling of H-3-bromohexine in rat urine and faeces",
abstract = "H-3-Bromohexine was dosed to rats as a model compound to allow comparison of HPLC-ICP-MS detection on bromine to radiochemical detection in an in vivo drug metabolism study. Metabolite profiles were obtained in urine and faeces extracts. No influence of the methanol gradient on the bromine response was observed in the range of 18 - 75% methanol. The sensitivity obtained by HPLC- ICP-MS was almost two orders of magnitude better than on-line H-3 radiochemical detection. For ICP- MS, the limit of detection was calculated to be 69 nM Br ( injection volume 100 mu l), corresponding to an absolute limit of detection of 1.3 ng of bromohexine on-column. This allowed ICP- MS detection of several minor metabolites that were not detected using radiochemical detection. Furthermore, metabolites that had lost the radioactive label were detected due to the bromine in the metabolites. As ICP- MS is also more selective than UV and molecular MS detection, it could thus be applied as an alternative detector in drug metabolism studies",
author = "B.P. Jensen and B. Gammelgaard and S.H. Hansen and J.V. Andersen",
year = "2005",
language = "English",
volume = "20",
pages = "204--209",
journal = "Journal of Analytical Atomic Spectrometry",
issn = "0267-9477",
publisher = "Royal Society of Chemistry",
number = "3",

}

RIS

TY - JOUR

T1 - HPLC-ICP-MS compared with radiochemical detection for metabolite profiling of H-3-bromohexine in rat urine and faeces

AU - Jensen, B.P.

AU - Gammelgaard, B.

AU - Hansen, S.H.

AU - Andersen, J.V.

PY - 2005

Y1 - 2005

N2 - H-3-Bromohexine was dosed to rats as a model compound to allow comparison of HPLC-ICP-MS detection on bromine to radiochemical detection in an in vivo drug metabolism study. Metabolite profiles were obtained in urine and faeces extracts. No influence of the methanol gradient on the bromine response was observed in the range of 18 - 75% methanol. The sensitivity obtained by HPLC- ICP-MS was almost two orders of magnitude better than on-line H-3 radiochemical detection. For ICP- MS, the limit of detection was calculated to be 69 nM Br ( injection volume 100 mu l), corresponding to an absolute limit of detection of 1.3 ng of bromohexine on-column. This allowed ICP- MS detection of several minor metabolites that were not detected using radiochemical detection. Furthermore, metabolites that had lost the radioactive label were detected due to the bromine in the metabolites. As ICP- MS is also more selective than UV and molecular MS detection, it could thus be applied as an alternative detector in drug metabolism studies

AB - H-3-Bromohexine was dosed to rats as a model compound to allow comparison of HPLC-ICP-MS detection on bromine to radiochemical detection in an in vivo drug metabolism study. Metabolite profiles were obtained in urine and faeces extracts. No influence of the methanol gradient on the bromine response was observed in the range of 18 - 75% methanol. The sensitivity obtained by HPLC- ICP-MS was almost two orders of magnitude better than on-line H-3 radiochemical detection. For ICP- MS, the limit of detection was calculated to be 69 nM Br ( injection volume 100 mu l), corresponding to an absolute limit of detection of 1.3 ng of bromohexine on-column. This allowed ICP- MS detection of several minor metabolites that were not detected using radiochemical detection. Furthermore, metabolites that had lost the radioactive label were detected due to the bromine in the metabolites. As ICP- MS is also more selective than UV and molecular MS detection, it could thus be applied as an alternative detector in drug metabolism studies

M3 - Journal article

VL - 20

SP - 204

EP - 209

JO - Journal of Analytical Atomic Spectrometry

JF - Journal of Analytical Atomic Spectrometry

SN - 0267-9477

IS - 3

ER -

ID: 44287858