Determination of methylphenidate in Calliphorid larvae by liquid-liquid extraction and liquid chromatography mass spectrometry - Forensic entomotoxicology using an in vivo rat brain model
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
The aim of this study was to examine the potential forensic utilisation of blowfly larvae (Diptera: Calliphoridae) as an alternative toxicological specimen for the detection of the psychotropic model drug methylphenidate (MPH). MPH was extracted from biological matrices (rat brain, serum and Calliphorid larvae) by liquid-liquid extraction with recovery of >80%, and quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LC-MS/MS assay was validated for entomotoxicological use and initially applied to male Sprague-Dawley rats (n=6) that were dosed with MPH (20mg/kg) ante-mortem. MPH could be detected in Calliphorid larvae (n=15) reared on the rat brains at 3.2±1.6ng/g. Secondly, MPH-spiked porcine brain tissue (450mg/kg) was used to investigate drug concentration in larvae over a period of 72h. After larvae had feed for 60h, MPH was detected at 19.8±1.4µg/g in the feeding larvae and at 3.5±0.1µg/g in the MPH-spiked porcine brain tissue. It could be advantageous to use Calliphorid larvae as an alternative toxicological specimen to detect alkaline labile drugs, such as MPH.
Originalsprog | Engelsk |
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Tidsskrift | Journal of Pharmaceutical and Biomedical Analysis |
Vol/bind | 70 |
Sider (fra-til) | 456-61 |
Antal sider | 6 |
ISSN | 0731-7085 |
DOI | |
Status | Udgivet - 2012 |
ID: 40849053