TY - JOUR

T1 - Comparison of direct injection nebulizer and desolvating microconcentric nebulizer for analysis of chlorine-, bromine- and iodine-containing compounds by reversed phase HPLC with ICP-MS detection

AU - Jensen, B.P.

AU - Gammelgaard, Bente

AU - Hansen, S.H.

AU - Andersen, J.V.

PY - 2003

Y1 - 2003

N2 - With the purpose of finding ways to combine micro-bore reversed phase HPLC with ICP-MS detection for analysis of drug substances containing chlorine, bromine and iodine, the suitability of a direct injection nebulizer and an Aridus desolvating microconcentric nebulizer was compared. Using the direct injection nebulizer at flow rates of 25 and 50 mul min(-1), the influence of 0-50% methanol and 0-25% acetonitrile on the sensitivity was studied. For chlorine and bromine, the relative sensitivity decreased with increasing amounts of organic solvent. For iodine, the relative sensitivity reached a maximum at low concentrations of organic solvent and then declined with increasing amounts of organic solvent. The response of the direct injection nebulizer was independent of the chemical structure of the analyte. For the desolvating microconcentric nebulizer, the response was dependent on the analyte structure. Many chlorine-, bromine-, and iodine-containing compounds were partially lost in the desolvating unit. For those chlorine- and iodine-containing compounds that were not lost, the sensitivity was independent of methanol concentration in the solvent when a 0-100% methanol gradient was applied

AB - With the purpose of finding ways to combine micro-bore reversed phase HPLC with ICP-MS detection for analysis of drug substances containing chlorine, bromine and iodine, the suitability of a direct injection nebulizer and an Aridus desolvating microconcentric nebulizer was compared. Using the direct injection nebulizer at flow rates of 25 and 50 mul min(-1), the influence of 0-50% methanol and 0-25% acetonitrile on the sensitivity was studied. For chlorine and bromine, the relative sensitivity decreased with increasing amounts of organic solvent. For iodine, the relative sensitivity reached a maximum at low concentrations of organic solvent and then declined with increasing amounts of organic solvent. The response of the direct injection nebulizer was independent of the chemical structure of the analyte. For the desolvating microconcentric nebulizer, the response was dependent on the analyte structure. Many chlorine-, bromine-, and iodine-containing compounds were partially lost in the desolvating unit. For those chlorine- and iodine-containing compounds that were not lost, the sensitivity was independent of methanol concentration in the solvent when a 0-100% methanol gradient was applied

M3 - Journal article

VL - 18

SP - 891

EP - 896

JO - Journal of Analytical Atomic Spectrometry

JF - Journal of Analytical Atomic Spectrometry

SN - 0267-9477

IS - 8

ER -