Comparison of different transition metal ions for immobilized metal affinity chromatography of selenoprotein P from human plasma
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Comparison of different transition metal ions for immobilized metal affinity chromatography of selenoprotein P from human plasma. / Sidenius, U; Farver, O; Jøns, O; Gammelgaard, Bente.
I: Journal of Chromatography B: Biomedical Sciences and Applications, Bind 735, Nr. 1, 1999, s. 85-91.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Comparison of different transition metal ions for immobilized metal affinity chromatography of selenoprotein P from human plasma
AU - Sidenius, U
AU - Farver, O
AU - Jøns, O
AU - Gammelgaard, Bente
PY - 1999
Y1 - 1999
N2 - Cu2+, Ni2+, Zn2+, Co2+ and Cd2+ were evaluated in metal ion affinity chromatography for enrichment of selenoprotein P, and immobilized Co2+ affinity chromatography was found to be the most selective chromatographic method. The chromatography was performed by fast protein liquid chromatography and the fractionation was followed by analysis of the collected fractions for selenium by inductively coupled plasma mass spectrometry. By the combination of immobilized Co2+ affinity chromatography and heparin affinity chromatography a simple method was developed yielding a 14,800-fold enrichment of selenoprotein P. The purity of the protein was determined by SDS-PAGE and by sequencing from polyvinylidene difluoride blots of SDS-PAGE gels.
AB - Cu2+, Ni2+, Zn2+, Co2+ and Cd2+ were evaluated in metal ion affinity chromatography for enrichment of selenoprotein P, and immobilized Co2+ affinity chromatography was found to be the most selective chromatographic method. The chromatography was performed by fast protein liquid chromatography and the fractionation was followed by analysis of the collected fractions for selenium by inductively coupled plasma mass spectrometry. By the combination of immobilized Co2+ affinity chromatography and heparin affinity chromatography a simple method was developed yielding a 14,800-fold enrichment of selenoprotein P. The purity of the protein was determined by SDS-PAGE and by sequencing from polyvinylidene difluoride blots of SDS-PAGE gels.
KW - Affinity Labels
KW - Amino Acid Sequence
KW - Cadmium
KW - Chromatography, Affinity
KW - Cobalt
KW - Copper
KW - Electrophoresis, Polyacrylamide Gel
KW - Humans
KW - Metals
KW - Molecular Weight
KW - Nickel
KW - Proteins
KW - Selenoprotein P
KW - Selenoproteins
KW - Sepharose
KW - Zinc
M3 - Journal article
C2 - 10630893
VL - 735
SP - 85
EP - 91
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 1387-2273
IS - 1
ER -
ID: 44053799