Rationale & Objective: YouTube is the number one internet-based video resource for any information. There are good number of videos available on antibiotic resistance, but the quality of the content is questionable. Hence, we aimed to assess the quality of those videos using a robust validated tool. Methodology: In Dec 2018 the term ‘Antibiotic Resistance‘ was searched on You Tube and first 76 videos were selected for our assessment.. All the video characteristics were collected and the quality of videos was assessed using Global Quality Scale. Two reviewers were independently involved in data collection and quality assessment and any disagreementwas resolved through discussion or consulting the third reviewer. SPSS v. 16 was used for descriptive statistics. Results: A total of 76 videos were evaluated out of the first appeared search. The majority (46.1%) of videos were educational videos and 52.6% were professional videos. The average time since the video available online was 2.67±2.042 years. The videos on antibiotic resistance accepted by the users with a good number of views, likes, and subscribers. The majority (42.11%) was graded as high, followed by low (38. 16%) and the remaining (19.74%) was moderate in quality. Moreover, the quality was not associated with category (≤0.228), animation status (≤0.347) and the relevancy (≤0.079) of the videos. Conclusion: Our analysis revealed that the quality of videos on antibiotics appeared to be inconclusive and acceptability of videos is not a measure of quality. Moreover, there is a huge need to produce high-quality videos from authentic resources and antibiotic societies.