We report on the fabrication of a microfluidic cell completely made of thiol-ene for microarray applications. To overcome the issues related to the hydrophobic nature of the thiol-ene material we have also introduced a new polymeric coating based of N, N-dimethylacrylamide (DMA) which is able to reduce the material hydrophobicity, to avoid aspecific protein binding and, thanks to the functional monomer propinyl mathacrylate, it is able to bind, in an oriented way, azido modified biomolecules.